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epha2 fc  (R&D Systems)


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    R&D Systems epha2 fc
    Epha2 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epha2+fc/Recombinant+Mouse+EphA2+Fc+Chimera+Protein%2C+CF/pm41692350-75-21-22
    Average 94 stars, based on 32 article reviews
    epha2 fc - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Molecular Determinants of EphA2 and EphB2 Antagonism Enable the Design of Ligands with Improved Selectivity.
    Article Snippet: .. 96- well ELISA high binding plates (Corning Costar, 9018) were incubated overnight at 4 °C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D System, 639-A2) diluted in sterile phosphatebuffered saline (PBS, 0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.65 g/L Na2HPO4, pH 7.4). ..

    Article Title: The rare bile acid isoallolithocholic acid (IALCA) is an EphA2 antagonist sparing FXR and TGR5 receptors.
    Article Snippet: .. Ninety-sixwells ELISA Costar® high-binding plate (Corning, NY, USA, #9018) was incubated overnight at 4 ◦C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D Systems, Minneapolis, MN, USA, #639A2) diluted in sterile PBS (0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.15 g/L Na2HPO4, pH 7.4). ..

    Article Title: Molecular Determinants of EphA2 and EphB2 Antagonism Enable the Design of Ligands with Improved Selectivity
    Article Snippet: Leupeptin, aprotinin, NP40, tween20, BSA, and salts for solution were from ITW Reagents (Chicago, Illinois); EDTA and sodium orthovanadate were from Merk (Darmstadt, Germany). .. 96-well ELISA high binding plates (Corning Costar, 9018) were incubated overnight at 4 °C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D System, 639-A2) diluted in sterile phosphate-buffered saline (PBS, 0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.65 g/L Na2HPO4, pH 7.4). ..

    Binding Assay:

    Article Title: Molecular Determinants of EphA2 and EphB2 Antagonism Enable the Design of Ligands with Improved Selectivity.
    Article Snippet: .. 96- well ELISA high binding plates (Corning Costar, 9018) were incubated overnight at 4 °C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D System, 639-A2) diluted in sterile phosphatebuffered saline (PBS, 0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.65 g/L Na2HPO4, pH 7.4). ..

    Article Title: Molecular Determinants of EphA2 and EphB2 Antagonism Enable the Design of Ligands with Improved Selectivity
    Article Snippet: Leupeptin, aprotinin, NP40, tween20, BSA, and salts for solution were from ITW Reagents (Chicago, Illinois); EDTA and sodium orthovanadate were from Merk (Darmstadt, Germany). .. 96-well ELISA high binding plates (Corning Costar, 9018) were incubated overnight at 4 °C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D System, 639-A2) diluted in sterile phosphate-buffered saline (PBS, 0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.65 g/L Na2HPO4, pH 7.4). ..

    Article Title: Supporting Information Design and Synthesis of Potent Bivalent Peptide Agonists Targeting the EphA2 Receptor
    Article Snippet: .. Molecular dynamics (MD) simulations were then performed using Sybyl x1.3 and Tripos force field for 200 ps while the temperature was maintained at 300 K. During the MD simulations, only residues of the EphA2 binding site and all residues of the SWL dimer (C6 linker) peptide were allowed to move, while the remaining residues were frozen at their positions in the crystal structure. peptides, 1 μg/ml EphA2 Fc (R&D System, Minneapolis MN) diluted in Tris-buffered saline (TBS; 50 mM Tris-HCl, 150 mM NaCl, pH 7.5) with 0.01% Tween 20 was used to coat Protein A coated 96 well plates (Pierce Thermo Scientific, Rockford, IL) for 1 hour at room temperature. ..

    Incubation:

    Article Title: Molecular Determinants of EphA2 and EphB2 Antagonism Enable the Design of Ligands with Improved Selectivity.
    Article Snippet: .. 96- well ELISA high binding plates (Corning Costar, 9018) were incubated overnight at 4 °C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D System, 639-A2) diluted in sterile phosphatebuffered saline (PBS, 0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.65 g/L Na2HPO4, pH 7.4). ..

    Article Title: The rare bile acid isoallolithocholic acid (IALCA) is an EphA2 antagonist sparing FXR and TGR5 receptors.
    Article Snippet: .. Ninety-sixwells ELISA Costar® high-binding plate (Corning, NY, USA, #9018) was incubated overnight at 4 ◦C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D Systems, Minneapolis, MN, USA, #639A2) diluted in sterile PBS (0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.15 g/L Na2HPO4, pH 7.4). ..

    Article Title: Ligands with different dimeric configurations potently activate the EphA2 receptor and reveal its potential for biased signaling
    Article Snippet: .. Briefly, EphA2 Fc (#639-A2, R&D Systems) was immobilized on protein A coated 96-well plates and then incubated with 0.05 nM ephrinA5 alkaline phosphatase (AP) and different peptide concentrations. .. The amount of bound ephrinA5 AP was quantified by using p-nitrophenyl phosphate substrate (#34045, Pierce-Thermo Scientific) diluted in SEAP buffer (105 mM diethanolamine, 0.5 mM MgCl 2 , pH 9.8) and optical density at 405 nm (OD 405 ) was measured.

    Article Title: Molecular Determinants of EphA2 and EphB2 Antagonism Enable the Design of Ligands with Improved Selectivity
    Article Snippet: Leupeptin, aprotinin, NP40, tween20, BSA, and salts for solution were from ITW Reagents (Chicago, Illinois); EDTA and sodium orthovanadate were from Merk (Darmstadt, Germany). .. 96-well ELISA high binding plates (Corning Costar, 9018) were incubated overnight at 4 °C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D System, 639-A2) diluted in sterile phosphate-buffered saline (PBS, 0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.65 g/L Na2HPO4, pH 7.4). ..

    Sterility:

    Article Title: Molecular Determinants of EphA2 and EphB2 Antagonism Enable the Design of Ligands with Improved Selectivity.
    Article Snippet: .. 96- well ELISA high binding plates (Corning Costar, 9018) were incubated overnight at 4 °C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D System, 639-A2) diluted in sterile phosphatebuffered saline (PBS, 0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.65 g/L Na2HPO4, pH 7.4). ..

    Article Title: The rare bile acid isoallolithocholic acid (IALCA) is an EphA2 antagonist sparing FXR and TGR5 receptors.
    Article Snippet: .. Ninety-sixwells ELISA Costar® high-binding plate (Corning, NY, USA, #9018) was incubated overnight at 4 ◦C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D Systems, Minneapolis, MN, USA, #639A2) diluted in sterile PBS (0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.15 g/L Na2HPO4, pH 7.4). ..

    Article Title: Molecular Determinants of EphA2 and EphB2 Antagonism Enable the Design of Ligands with Improved Selectivity
    Article Snippet: Leupeptin, aprotinin, NP40, tween20, BSA, and salts for solution were from ITW Reagents (Chicago, Illinois); EDTA and sodium orthovanadate were from Merk (Darmstadt, Germany). .. 96-well ELISA high binding plates (Corning Costar, 9018) were incubated overnight at 4 °C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D System, 639-A2) diluted in sterile phosphate-buffered saline (PBS, 0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.65 g/L Na2HPO4, pH 7.4). ..

    Saline:

    Article Title: Molecular Determinants of EphA2 and EphB2 Antagonism Enable the Design of Ligands with Improved Selectivity.
    Article Snippet: .. 96- well ELISA high binding plates (Corning Costar, 9018) were incubated overnight at 4 °C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D System, 639-A2) diluted in sterile phosphatebuffered saline (PBS, 0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.65 g/L Na2HPO4, pH 7.4). ..

    Article Title: Molecular Determinants of EphA2 and EphB2 Antagonism Enable the Design of Ligands with Improved Selectivity
    Article Snippet: Leupeptin, aprotinin, NP40, tween20, BSA, and salts for solution were from ITW Reagents (Chicago, Illinois); EDTA and sodium orthovanadate were from Merk (Darmstadt, Germany). .. 96-well ELISA high binding plates (Corning Costar, 9018) were incubated overnight at 4 °C with 100 μL/well of 1 μg/mL EphA2-Fc (R&D System, 639-A2) diluted in sterile phosphate-buffered saline (PBS, 0.2 g/L KCl, 8.0 g/L NaCl, 0.2 g/L KH2PO4, 1.65 g/L Na2HPO4, pH 7.4). ..

    Article Title: Supporting Information Design and Synthesis of Potent Bivalent Peptide Agonists Targeting the EphA2 Receptor
    Article Snippet: .. Molecular dynamics (MD) simulations were then performed using Sybyl x1.3 and Tripos force field for 200 ps while the temperature was maintained at 300 K. During the MD simulations, only residues of the EphA2 binding site and all residues of the SWL dimer (C6 linker) peptide were allowed to move, while the remaining residues were frozen at their positions in the crystal structure. peptides, 1 μg/ml EphA2 Fc (R&D System, Minneapolis MN) diluted in Tris-buffered saline (TBS; 50 mM Tris-HCl, 150 mM NaCl, pH 7.5) with 0.01% Tween 20 was used to coat Protein A coated 96 well plates (Pierce Thermo Scientific, Rockford, IL) for 1 hour at room temperature. ..

    Control:

    Article Title: Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells
    Article Snippet: Briefly, coverslips (diameter, 15 mm) were incubated overnight with poly-L-ornithine in PBS (100 μL of 100 μg/mL; P2533, Sigma-Aldrich), washed with sterile water, and dried. .. Comb-shaped silicon masks containing parallel teeth and gaps (each approximately 0.48 mm wide) were applied on the glass surface, and then 100 μL of 10 μg/mL ephrin-A1-Fc (602-A1, R&D Systems), EphA2-Fc (639-A2, R&D Systems), ephrin-B1-Fc (473-EB, R&D System), EphB4-Fc (446-E4, R&D System), or human IgG Fc as a control (Fc; 55911, MP Biomedicals) were adsorbed onto the surface at 37 °C for 60 min. ..

    Article Title: Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells.
    Article Snippet: Briefly, coverslips (diameter, 15 mm) were incubated overnight with poly-L-ornithine in PBS (100 μL of 100 μg/mL; P2533, SigmaAldrich), washed with sterile water, and dried. .. Comb-shaped silicon masks containing parallel teeth and gaps (each approximately 0.48 mm wide) were applied on the glass surface, and then 100 μL of 10 μg/mL ephrin-A1-Fc (602-A1, R&D Systems), EphA2-Fc (639-A2, R&D Systems), ephrin-B1-Fc (473-EB, R&D System), EphB4-Fc (446-E4, R&D System), or human IgG Fc as a control (Fc; 55911, MP Biomedicals) were adsorbed onto the surface at 37 ◦C for 60 min. ..



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    Image Search Results


    Physicochemical and in vitro characterization data of BCY18469. a Human plasma ; b Mouse plasma, c Human EphA2, d  Mouse EphA2

    Journal: Theranostics

    Article Title: Development and preclinical characterization of a novel radiotheranostic EphA2-targeting bicyclic peptide

    doi: 10.7150/thno.96641

    Figure Lengend Snippet: Physicochemical and in vitro characterization data of BCY18469. a Human plasma ; b Mouse plasma, c Human EphA2, d Mouse EphA2

    Article Snippet: Mouse proteins: Mouse EphA2 was purchased from R&D Systems (Product number 639-A2).

    Techniques: In Vitro, Clinical Proteomics, Protein Binding